7DGX

Structure of coiled coil domain of Trypanosoma brucei coronin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	273
Detector technology	CCD
Collection date	2018-06-10
Detector	MAR CCD 130 mm
Wavelength(s)	0.987
Spacegroup name	I 4 2 2
Unit cell lengths	93.376, 93.376, 82.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.060 - 2.060
R-factor	0.228
Rwork	0.227
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cx2
RMSD bond length	0.001
RMSD bond angle	0.192
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.060	2.130
High resolution limit [Å]	2.056	2.060
Number of reflections	11685	1145
<I/σ(I)>	1.59
Completeness [%]	99.0
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	3M NaCl, 0.1 M HEPESE 7.5