7DF7

Crystal structure of human V-1 in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	49.620, 56.280, 81.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.240 - 2.300
R-factor	0.2197
Rwork	0.218
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aaa
RMSD bond length	0.003
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.386	46.386	2.360
High resolution limit [Å]	2.300	10.280	2.300
Rmerge	0.071	0.027	1.228
Rmeas	0.074	0.029	1.284
Number of reflections	10568	147	757
<I/σ(I)>	21.83	63.48	2.13
Completeness [%]	99.8	99.3	97.4
Redundancy	12.82	10.571	11.77
CC(1/2)	1.000	1.000	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 mM ammonium acetate, 20% PEG 3350