7DEY

Structure of Dicer from Pichia stipitis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-21
Detector	RAYONIX MX300-HS
Wavelength(s)	0.987
Spacegroup name	P 43
Unit cell lengths	127.738, 127.738, 103.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.682 - 2.897
R-factor	0.2374
Rwork	0.235
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oog
RMSD bond length	0.009
RMSD bond angle	1.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.897	2.900
Number of reflections	37041	1824
<I/σ(I)>	20.52
Completeness [%]	99.0
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	3.6 M NaCl and 0.1 M sodium acetate (pH 5.4-5.6)