7DER

Lysozyme alone in H2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	78.777, 78.777, 37.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.559 - 1.030
R-factor	0.1495
Rwork	0.149
R-free	0.17330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wld
RMSD bond length	0.006
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.559	1.056
High resolution limit [Å]	1.030	1.030
Rmerge	0.073	0.728
Number of reflections	58098	3926
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	12.2
CC(1/2)	0.998	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	15mg/mL lysozyme, 0.7M NaCl in a buffer of 50mM sodium-acetate (pH4.5)