7DCW

The structure of the Arabidopsis thaliana guanosine deaminase complexed with adenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	277
Detector technology	CCD
Collection date	2019-10-20
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	119.084, 119.084, 39.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.630 - 2.300
R-factor	0.1796
Rwork	0.177
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.635	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.243	0.934
Number of reflections	14618	2121
<I/σ(I)>	10.5	2.6
Completeness [%]	99.9	100
Redundancy	8.3	6.8
CC(1/2)	0.988	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)