7DCH
Alpha-glucosidase from Weissella cibaria BBK-1 bound with acarbose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2019-06-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 116.992, 116.992, 107.167 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.755 - 1.692 |
Rwork | 0.177 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d9b |
RMSD bond length | 0.009 |
RMSD bond angle | 1.081 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (7.0.069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmeas | 0.077 | |
Rpim | 0.030 | 0.416 |
Number of reflections | 76028 | 15816 |
<I/σ(I)> | 21.244 | |
Completeness [%] | 91.4 | |
Redundancy | 6.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium sulfate, dioxane, MES |