7DC3

Crystal structure of the MyRF ICA domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 3 2 1
Unit cell lengths	78.706, 78.706, 138.464
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.220 - 2.400
Rwork	0.217
R-free	0.26790
Structure solution method	SIRAS
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.19rc3_4028)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.220	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	20050	1965
<I/σ(I)>	16.78
Completeness [%]	96.0	69.9
Redundancy	18.2	13.2
CC(1/2)	0.971	0.029

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 6000, Lithium chloride, MES