7DC1
Crystal structure of human copper homeostatic proteins atox1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.493, 104.493, 29.188 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.473 - 1.750 |
| Rwork | 0.181 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f0w |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.473 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.056 | 0.616 |
| Number of reflections | 18311 | 1002 |
| <I/σ(I)> | 17 | 2.3 |
| Completeness [%] | 98.5 | 97.1 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M LiCl, 0.1M Tris pH8, 20% PEG6,000 |
