7DBU
Crystal structure of catalytic domain of Anhydrobiosis-related Mn-dependent Peroxidase (AMNP) from Ramazzottius varieornatus (Zn2+-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-08-29 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.1200 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.293, 48.537, 49.034 |
| Unit cell angles | 73.25, 68.61, 89.95 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.933 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.112 | 1.039 |
| Number of reflections | 40100 | 956 |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 90.1 | 48.4 |
| Redundancy | 7.9 | 7.2 |
| CC(1/2) | 0.997 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG3350, 100 mM imidazole-HCl buffer (pH 7.5), 300 mM zinc acetate, and 50 mM sodium fluoride |
