7DBC
PRA in complex with tubulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.417, 158.270, 180.747 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.923 - 2.400 |
R-factor | 0.1986 |
Rwork | 0.198 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.923 | 19.920 | 2.440 |
High resolution limit [Å] | 2.400 | 13.150 | 2.400 |
Rmerge | 0.112 | 0.068 | 0.858 |
Rmeas | 0.122 | 0.074 | 0.930 |
Rpim | 0.047 | 0.030 | 0.357 |
Total number of observations | 3321 | 38700 | |
Number of reflections | 118342 | 573 | 5789 |
<I/σ(I)> | 10.3 | 21 | 2.2 |
Completeness [%] | 99.8 | 71.4 | 100 |
Redundancy | 6.8 | 5.8 | 6.7 |
CC(1/2) | 0.995 | 0.991 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7 |