7DBB

SSE in complex with tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	105.000, 157.573, 181.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.877 - 2.805
R-factor	0.1741
Rwork	0.172
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.171	0.046	1.060
Rmeas	0.178	0.048	1.102
Rpim	0.049	0.014	0.300
Total number of observations	975739
Number of reflections	74418	3989	3681
<I/σ(I)>	2.6
Completeness [%]	100.0	99.8	100
Redundancy	13.1	12.2	13.2
CC(1/2)		0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7