7DB5

Crystal structure of alpha-L-fucosidase from Vibrio sp. strain EJY3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 63
Unit cell lengths	163.916, 163.916, 58.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.500 - 1.900
R-factor	0.1723
Rwork	0.171
R-free	0.20330
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.837
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.075	0.058	0.328
Rmeas	0.081	0.063	0.352
Rpim	0.029	0.023	0.126
Total number of observations	515865
Number of reflections	67820	3320	3433
<I/σ(I)>	12.8
Completeness [%]	95.3	89.3	96.8
Redundancy	7.6	7.2	7.6
CC(1/2)		0.995	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Sodium dihydrogen phosphate, Potassium hydrogen phosphate, acetate