7DB4
Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in TDP012- and glutathione-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.675, 75.133, 108.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.900 - 1.540 |
R-factor | 0.1682 |
Rwork | 0.167 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kem |
RMSD bond length | 0.004 |
RMSD bond angle | 1.096 |
Data reduction software | XDS (17, 2015) |
Data scaling software | Aimless (0.5.15) |
Phasing software | MOLREP (11.7.02) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.900 | 1.597 |
High resolution limit [Å] | 1.540 | 1.542 |
Rmerge | 0.043 | 0.399 |
Rmeas | 0.047 | |
Number of reflections | 71336 | 6890 |
<I/σ(I)> | 26.52 | |
Completeness [%] | 99.7 | 97.92 |
Redundancy | 7 | 5.2 |
CC(1/2) | 1.000 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 34% (v/v) PPG400 in 80mM Bis-Tris, pH 6.4 |