7D9Z
Crystal structure of anti-basigin Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.452, 53.048, 54.313 |
| Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
| Resolution | 36.400 - 1.123 |
| R-factor | 0.1223 |
| Rwork | 0.121 |
| R-free | 0.15030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ma3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | pointless (1.12.2) |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | PHENIX (1.19rc1_4016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.924 | 1.194 |
| High resolution limit [Å] | 1.123 | 1.123 |
| Number of reflections | 139276 | 6905 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 81.6 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.999 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1% n-Octyl-beta-D-glucoside, 0.1M sodium citrate tribasic dihydrate, 22% PEG 3350 |
