7D9X
Highly active mutant W525D of Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.624, 117.189, 187.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.900 - 1.740 |
| R-factor | 0.1673 |
| Rwork | 0.167 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zjg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.847 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 1.790 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.090 | 1.050 |
| Number of reflections | 111797 | 5485 |
| <I/σ(I)> | 47.9 | 2.7 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 16% PEG 8000, 15% PEG 400, 0.1M HEPES pH 7, 50mM Gly-Gly |
