7D9I
SpdH Spermidine dehydrogenase D282A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.273, 85.762, 100.040 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 39.350 - 2.100 |
| R-factor | 0.1591 |
| Rwork | 0.156 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d9f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.932 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.085 | 0.404 |
| Number of reflections | 67104 | 3334 |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Bis Tris pH6.5, 20% w/v polyethylene glycol (PEG) 3350 |
