7D8U

Crystal structure of the C-terminal domain of pNP868R from African swine fever virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9788
Spacegroup name	P 65 2 2
Unit cell lengths	127.315, 127.315, 208.198
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.068 - 2.700
R-factor	0.187
Rwork	0.184
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ri1
RMSD bond length	0.011
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.131	0.752
Rmeas	0.133	0.766
Rpim	0.022	0.145
Number of reflections	28114	1364
<I/σ(I)>	27	3
Completeness [%]	100.0	99.7
Redundancy	35.9	25.8
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	4.3M sodium formate