7D8T

MITF bHLHLZ complex with M-box DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	207.260, 207.260, 163.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.791 - 3.201
R-factor	0.2057
Rwork	0.204
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d8s
RMSD bond length	0.005
RMSD bond angle	0.648
Data reduction software	HKL-2000
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	120.734	48.158	3.370
High resolution limit [Å]	3.200	10.120	3.200
Rmerge		0.076	0.901
Rmeas	0.140	0.081	0.944
Rpim	0.042	0.026	0.281
Total number of observations	251159	7249	36598
Number of reflections	22309	746	3223
<I/σ(I)>	10.6	26.6	2.6
Completeness [%]	100.0	99.1	100
Redundancy	11.3	9.7	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG4000, Tris-Cl pH8.5, sodium citrate.