7D8Q
The structure of nucleotide phosphatase Sa1684 complex with GDP analogue from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.659, 36.124, 58.924 |
| Unit cell angles | 90.00, 111.46, 90.00 |
Refinement procedure
| Resolution | 30.190 - 1.500 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18600 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.193 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | |
| Number of reflections | 30428 | 1505 |
| <I/σ(I)> | 29.8 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20%PEG400, 0.1M sodium citrate pH 5.5 |
