7D8C
Crystal structure of the Cas12i1-crRNA binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 211.933, 211.933, 164.758 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.420 - 3.600 |
R-factor | 0.2815 |
Rwork | 0.280 |
R-free | 0.30850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d2l |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHENIX (1.13-2998) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.420 | 3.850 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 25821 | 4599 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 99.9 | |
Redundancy | 38.6 | |
CC(1/2) | 0.998 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium citrate (pH 5.2), 17% (w/v) Polyethylene glycol 3350, 2% Tacsimate (pH 4.0), 0.15 M sodium citrate tribasic dihydrate |