7D8A
Crystal Structure of H3(1-13)/PHF14-PZP fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.2818 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.781, 150.423, 81.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.177 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d86 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 2.000 |
| Number of reflections | 33740 | 1282 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 93.9 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Hepes pH 7.5, 18% PEG4000, 12% isopropanol . |
