7D86
Crystal Structure of zebrafishPHF14-PZP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.2818 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.193, 41.907, 52.878 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 41.680 - 1.840 |
| R-factor | 0.1793 |
| Rwork | 0.174 |
| R-free | 0.22460 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.840 | 5.160 | 1.900 |
| Rmerge | 0.098 | 0.078 | 0.703 |
| Rmeas | 0.110 | 0.088 | 0.796 |
| Rpim | 0.050 | 0.040 | 0.364 |
| Number of reflections | 29554 | 764 | 733 |
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 98.3 | 97.2 | 98.3 |
| Redundancy | 4.3 | 4.2 | 4.3 |
| CC(1/2) | 0.991 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Magnesium chloride, 0.1 M Bis-Tris, 25 % v/v PEG 3350 |
