7D7U
Crystal structure of Ago2 MID domain in complex with 8-Br-adenosin-5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.170, 65.770, 40.790 |
| Unit cell angles | 105.83, 96.46, 87.56 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2067 |
| Rwork | 0.204 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lud |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.310 | 31.310 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.107 | 0.022 | 0.758 |
| Rmeas | 0.151 | 0.031 | 1.072 |
| Rpim | 0.107 | 0.022 | 0.758 |
| Total number of observations | 58839 | 601 | 4429 |
| Number of reflections | 29976 | 311 | 2252 |
| <I/σ(I)> | 4.2 | 16.6 | 0.8 |
| Completeness [%] | 94.6 | 88.4 | 93.9 |
| Redundancy | 2 | 1.9 | 2 |
| CC(1/2) | 0.989 | 0.998 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 0.1M imidazole pH8.0, 0.2M NaCl, 0.46M NaH2PO4, 1.84M K2HPO4 |
