7D7O

Crystal structure of cystathionine gamma-lyase from Bacillus cereus ATCC 14579

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	116.708, 116.708, 147.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.040 - 1.980
R-factor	0.2147
Rwork	0.212
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6khq
RMSD bond length	0.008
RMSD bond angle	1.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.980	1.980
Number of reflections	71253	3186
<I/σ(I)>	35.02
Completeness [%]	93.7
Redundancy	11.8
CC(1/2)	1.000	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	2M Ammonium sulfate, 0.1 M Tris-HCl (pH 8.5)