7D79
The structure of DcsB complex with its substrate analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.263, 59.011, 113.990 |
| Unit cell angles | 84.85, 78.55, 84.01 |
Refinement procedure
| Resolution | 39.003 - 2.104 |
| R-factor | 0.184609801223 |
| Rwork | 0.182 |
| R-free | 0.22694 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.489 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Number of reflections | 64129 | 64129 |
| <I/σ(I)> | 9.56 | |
| Completeness [%] | 92.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.750 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.2 | 0.1M MMT, 25% PEG 1500 |
