7D6W

Crystal structure of Phycocyanin from Synechococcus sp. R42DM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	80
Detector technology	CCD
Collection date	2019-10-04
Detector	MARRESEARCH
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	106.630, 113.990, 183.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.842 - 2.150
Rwork	0.165
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h0m
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.842	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.124	0.384
Number of reflections	122052	5976
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.994	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M HEPES pH 6.5, 10% (W/V) PEG 6000