7D6A
Crystal structure of Oryza sativa Os4BGlu18 monolignol beta-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.134, 83.759, 207.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.1514 |
| Rwork | 0.150 |
| R-free | 0.17520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rgl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.379 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 96808 | 9639 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 96.0 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | 19% PEG 3350, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 288 K |
