7D5Z
Crystal structure of EBV gH/gL bound with neutralizing antibody 1D8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 212.865, 212.865, 598.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.030 - 4.200 |
| R-factor | 0.2474 |
| Rwork | 0.246 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t1d |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.080 | 4.350 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.131 | |
| Number of reflections | 100776 | 9853 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.997 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200 mM Sodium Citrate, 100 mM HEPES Sodium Salt, pH 7.5, 15 % w/v MPD |
