7D4E

Structure of L-lysine oxidase precursor in complex with L-lysine (1.0 M)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	94.889, 130.050, 94.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.025 - 1.850
R-factor	0.1669
Rwork	0.165
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3x0v
RMSD bond length	0.019
RMSD bond angle	1.631
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.700	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.048	0.322
Rpim	0.028	0.186
Number of reflections	49629	3015
<I/σ(I)>	16.9	4
Completeness [%]	99.3	99
Redundancy	4.4	4.5
CC(1/2)	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris-HCl pH8.0 (-9.0) and 2.1 M ammonium dihydrogen phosphate