7D4C
Structure of L-lysine oxidase precursor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.160, 130.920, 94.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.975 - 1.970 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x0v |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.821 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.100 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.106 | 0.527 |
| Rpim | 0.080 | 0.284 |
| Number of reflections | 39115 | 2880 |
| <I/σ(I)> | 10.3 | 2.9 |
| Completeness [%] | 93.5 | 99.9 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.978 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH8.5 and 2.0 M ammonium dihydrogen phosphate |
