7D40
Crystal structure of GATase subunit of Methanocaldococcus jannaschii GMP synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-08-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.280, 65.280, 97.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.530 - 1.670 |
| R-factor | 0.1693 |
| Rwork | 0.167 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wl8 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.137 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.530 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.112 | |
| Number of reflections | 52939 | 42843 |
| <I/σ(I)> | 4.27 | |
| Completeness [%] | 99.4 | 83.6 |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 277 | 100 mM sodium acetate, pH 5.6, 35 % PEG 6000 |
