7D3T

Crystal structure of OSPHR2 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97854
Spacegroup name	P 1 21 1
Unit cell lengths	57.414, 100.824, 79.351
Unit cell angles	90.00, 102.46, 90.00

Refinement procedure

Resolution	49.000 - 2.700
R-factor	0.2151
Rwork	0.213
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kvo
RMSD bond length	0.011
RMSD bond angle	1.377
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.070
Rmeas	0.084
Rpim	0.060	0.691
Number of reflections	45458	11093
<I/σ(I)>	12	1.7
Completeness [%]	99.2
Redundancy	3.4
CC(1/2)	0.997	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, sodium malonate