7D35

Human LC8 bound to ebola virus VP35(67-76)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2019-12-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 61 2 2
Unit cell lengths	43.426, 43.426, 205.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.330 - 2.401
R-factor	0.234
Rwork	0.228
R-free	0.29170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cmi
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.094	0.310
Number of reflections	4869	225
<I/σ(I)>	17.2
Completeness [%]	95.0
Redundancy	4.7
CC(1/2)	0.975	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM ammonium citrate dibasic, 200mM ammonium citrate tribasic (pH 5.5), 30% (w/v) polyethylene glycol 3350