7D2W
Crystal structure of PHIT/PHISTa-like subfamily PF3D7_1372300 protein from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.523, 78.523, 100.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.430 - 1.970 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.20400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 24930 | 2472 |
| <I/σ(I)> | 45.45 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20.5 |
| CC(1/2) | 1.000 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 16% PEG 8000, 50mM KH2PO4 |
