7D2L
Crystal structure of the Cas12i1 R-loop complex before target DNA cleavage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 129.192, 142.644, 209.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.030 - 2.750 |
R-factor | 0.2154 |
Rwork | 0.213 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the selenomethionine (SeMet)-labeled complex |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHENIX (1.13-2998) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.030 | 2.840 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.070 | 0.761 |
Number of reflections | 50543 | 4567 |
<I/σ(I)> | 24.3 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.998 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M sodium citrate (pH5.5), PEG3350 (18%), 0.1M sodium citrate |