7D2C

The Crystal Structure of human PARP14 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97936
Spacegroup name	P 21 21 21
Unit cell lengths	33.761, 41.281, 109.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.750 - 1.560
Rwork	0.175
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5qi5
RMSD bond length	0.005
RMSD bond angle	1.235
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.766	1.590
High resolution limit [Å]	1.560	1.560
Rmerge	0.053	0.137
Number of reflections	22373	1006
<I/σ(I)>	23.1
Completeness [%]	99.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaCl, 0.1M Tris pH8.5, 25% PEG3350