7D1L

complex structure of two RRM domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	54.686, 86.908, 92.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.653 - 1.950
R-factor	0.1868
Rwork	0.184
R-free	0.22470
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.143	1.075
Number of reflections	58993	3258
<I/σ(I)>	19.2
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	0.994	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2% v/v 1, 4-Dioxane 0.1M Tris pH 8.0 15% w/v PEG3350 0.01 M Nickel(II) Chloride hexahydrate