7D1I

Crystal structure of acinetobacter baumannii MurG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	104.15
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	182.909, 182.909, 156.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.259 - 3.487
Rwork	0.261
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f0k
RMSD bond length	0.017
RMSD bond angle	2.050
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.264	3.570
High resolution limit [Å]	3.487	3.487
Number of reflections	31262	1765
<I/σ(I)>	9.45
Completeness [%]	90.8
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris pH8.8, 2.6M Sodium chloride 0.15M Ca(OAc)2