7D18
Crystal structure of Acidobacteriales bacterium glutaminyl cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.044, 78.330, 44.892 |
| Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
| Resolution | 25.909 - 1.332 |
| R-factor | 0.1151 |
| Rwork | 0.114 |
| R-free | 0.14030 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.107 | 0.775 |
| Number of reflections | 53659 | 5037 |
| <I/σ(I)> | 28 | 3.9 |
| Completeness [%] | 98.5 | 92.6 |
| Redundancy | 10 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M (NH4)2SO4, 0.1 M sodium cacodylate, pH 6.5, 30% (w/v) PEG 8000 |
