7D0M

Crystal structure of mouse CRY1 with bound cryoprotectant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	45.057, 77.507, 134.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.250 - 1.950
R-factor	0.1787
Rwork	0.177
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kx4
RMSD bond length	0.004
RMSD bond angle	0.707
Data reduction software	xia2 (0.6.467)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.250	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.139	0.496
Rmeas	0.153	0.544
Rpim	0.062	0.220
Number of reflections	35031	5000
<I/σ(I)>	8.2	3.2
Completeness [%]	99.5	99.2
Redundancy	6	6.1
CC(1/2)	0.994	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.25 M NH4Cl, 22% w/v PEG 3350, 3% v/v ethylene glycol