7CZJ

The Crystal Structure of Family 20 CBM of Maltotetraose-forming Amylase from Pseudomonas Saccharophila STB07

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978910
Spacegroup name	P 21 21 21
Unit cell lengths	40.413, 79.883, 92.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.495
R-factor	0.1866
Rwork	0.185
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0b
RMSD bond length	0.008
RMSD bond angle	1.746
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.742	2.000
High resolution limit [Å]	1.495	1.495
Rmerge	0.073
Number of reflections	48893	48893
<I/σ(I)>	14.9
Completeness [%]	99.2
Redundancy	6.7
CC(1/2)		0.073

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293.15	0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dehydrate. 1.0 M lithium sulfate monohydrate