7CZJ
The Crystal Structure of Family 20 CBM of Maltotetraose-forming Amylase from Pseudomonas Saccharophila STB07
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.978910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.413, 79.883, 92.655 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.750 - 1.495 |
R-factor | 0.1866 |
Rwork | 0.185 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z0b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.746 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.742 | 2.000 |
High resolution limit [Å] | 1.495 | 1.495 |
Rmerge | 0.073 | |
Number of reflections | 48893 | 48893 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 99.2 | |
Redundancy | 6.7 | |
CC(1/2) | 0.073 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.15 | 0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dehydrate. 1.0 M lithium sulfate monohydrate |