7CZH
PL24 ulvan lyase-Uly1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.100, 99.444, 94.996 |
| Unit cell angles | 90.00, 90.55, 90.00 |
Refinement procedure
| Resolution | 47.496 - 2.104 |
| R-factor | 0.1578 |
| Rwork | 0.156 |
| R-free | 0.19410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.104 | 2.104 |
| Rmerge | 0.219 | 0.337 |
| Rmeas | 0.237 | 0.365 |
| Number of reflections | 62242 | 3092 |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM calcium acetate hydrate (pH 6.5), 18% (w/v) polyethylene glycol (PEG) 8000, and 100 mM sodium cacodylate trihydrate |
