7CZC

Crystal structure of apo-FabG from Vibrio harveyi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-02-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	58.459, 91.320, 94.158
Unit cell angles	90.00, 103.22, 90.00

Refinement procedure

Resolution	19.749 - 2.000
R-factor	0.2106
Rwork	0.209
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i01
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.750	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	65055	6451
<I/σ(I)>	21.02
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	0.15M DL-malic acid (pH 7.2) , 20% w/v PEG 3350