7CYX

Crystal strcuture of Glycine oxidase from Bacillus cereus ATCC 14579

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	297
Detector technology	CCD
Collection date	2020-05-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	82.180, 132.810, 165.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.410
R-factor	0.2268
Rwork	0.223
R-free	0.29990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ysh
RMSD bond length	0.009
RMSD bond angle	1.416
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.450
High resolution limit [Å]	2.360	6.520	2.410
Rmerge	0.102	0.061	1.358
Rmeas	0.107	0.064	1.407
Rpim	0.031	0.021	0.366
Number of reflections	36198	1792	1802
<I/σ(I)>	11
Completeness [%]	98.8	90.9	100
Redundancy	12.9	9.6	14.6
CC(1/2)		0.995	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Tacsimate, HEPES, PEG 3350