7CXV

Crystal structure of CmnK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9732
Spacegroup name	P 2 21 21
Unit cell lengths	50.436, 87.264, 147.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.140 - 2.350
R-factor	0.2009
Rwork	0.199
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cxs
RMSD bond length	0.005
RMSD bond angle	1.371
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	24261	2568
<I/σ(I)>	26.42	4.68
Completeness [%]	95.4
Redundancy	5.8
CC(1/2)	0.980	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50 mM MgCl2.6H2O, 30% w/v PEG-550, 1 mM L-Dap, and 100 mM HEPES