7CXS
Crystal structure of CmnK, a L-Dap formation enzyme in capreomycin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 50.106, 87.080, 146.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.973 - 1.830 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mp3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.421 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 56430 | 5363 |
| <I/σ(I)> | 42.6 | |
| Completeness [%] | 98.6 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.990 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM MgCl2.6H2O, 30% w/v PEG-550, and 100 mM HEPES |
