7CWY

Crystal structure of a tyrosine decarboxylase from Enterococcus faecalis in complex with the cofactor PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	P 61 2 2
Unit cell lengths	132.527, 132.527, 390.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.950 - 2.590
R-factor	0.1985
Rwork	0.196
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hsi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.680
High resolution limit [Å]	2.590	5.560	2.590
Rmerge	0.100	0.041	0.847
Rmeas	0.105	0.044	0.889
Rpim	0.031	0.014	0.266
Total number of observations	730013
Number of reflections	63827	6792	6256
<I/σ(I)>	9.9
Completeness [%]	99.3	98.1	99.3
Redundancy	11.4	10.1	11.1
CC(1/2)		0.999	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	15% PEG 1000, 0.2 M Mgcl2 and 0.1 M sodium citrate tribasic dehydrate (pH 6.5)