7CWW

Crystal structure of TsrL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	75.895, 78.118, 78.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.730 - 2.000
R-factor	0.1721
Rwork	0.170
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w98
RMSD bond length	0.007
RMSD bond angle	0.803
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Number of reflections	32054	32045
<I/σ(I)>	22.3
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	0.996	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.09 M NPS, 50% (v/v) precipitant Mix 1, 0.1 M buffer system 3 at pH 8.5 from the Morpheus screen kit of Molecular Dimension company