7CWJ

Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	RAYONIX MX340-HS
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	45.658, 67.478, 170.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.952 - 1.610
R-factor	0.1532
Rwork	0.151
R-free	0.19950
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	RESOLVE
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.100	44.100	1.630
High resolution limit [Å]	1.600	8.770	1.600
Rmerge	0.067	0.042	0.416
Rmeas	0.070	0.044	0.451
Rpim	0.019	0.013	0.169
Total number of observations	818842
Number of reflections	67496	523	2510
<I/σ(I)>	27.5		3.5
Completeness [%]	96.2	99.6	73.2
Redundancy	12.1	10.7	6.8
CC(1/2)	0.999	0.999	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.9 M sodium malonate pH 6.5, 5% glycerol