7CVM

Crystal structure of glucose isomerase by fixed-target serial synchrotron crystallography (500 ms)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	I 2 2 2
Unit cell lengths	94.550, 100.260, 103.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.050 - 2.000
R-factor	0.2156
Rwork	0.214
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y4i
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.460	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	33589	3282
<I/σ(I)>	2.05	1.52
Completeness [%]	100.0
Redundancy	30.8
CC(1/2)	0.707	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate